Publications
Structural insights into a broadly reactive nanobody that binds pathogenic and non-pathogenic lagoviruses
Derin Kara, Marie Pancera, Gholamreza Hassanzadeh-Ghassabeh, Steve Schoonooghe, Veronika Masic, Lauren Hartley-Tassell, Biswa Prasanna Mishra, Thomas Ve, Thomas Haselhorst, Mark von Itzstein, Grant S. Hansman
Journal of Virology 2026
Q586B2 is a crucial virulence factor during the early stages of Trypanosoma brucei infection that is conserved amongst trypanosomatids
Benoit Stijlemans, Patrick De Baetselier, Inge Van Molle, Laurence Lecordier, Erika Hendrickx, Ema Romão, Cécile Vincke, Wendy Baetens, Steve Schoonooghe, Gholamreza Hassanzadeh-Ghassabeh, Hannelie Korf, Marie Wallays, Joar E. Pinto Torres, David Perez-Morga, Lea Brys, Oscar Campetella, María S. Leguizamón, Mathieu Claes, Sarah Hendrickx, Dorien Mabille, Guy Caljon, Han Remaut, Kim Roelants, Stefan Magez, Jo A. Van Ginderachter & Carl De Trez
Nature Communications volume 15, 1779, 2024
NMR-Based Analysis of Nanobodies to SARS-CoV-2 Nsp9 Reveals a Possible Antiviral Strategy Against COVID-19
Esposito G, Hunashal Y, Percipalle M, Venit T, Dieng MM, Fogolari F, Hassanzadeh G, Piano F, Gunsalus KC, Idaghdour Y, Percipalle P.
Adv Biol (Weinh). 2021 Dec;5(12):e2101113
An affinity-enhanced, broadly neutralizing heavy chain-only antibody protects against SARS-CoV-2 infection in animal models
Schepens B, van Schie L, Nerinckx W, Roose K, Van Breedam W, Fijalkowska D, Devos S, Weyts W, De Cae S, Vanmarcke S, Lonigro C, Eeckhaut H, Van Herpe D, Borloo J, Oliveira AF, Catani JPP, Creytens S, De Vlieger D, Michielsen G, Marchan JCZ, Moschonas GD, Rossey I, Sedeyn K, Van Hecke A, Zhang X, Langendries L, Jacobs S, Ter Horst S, Seldeslachts L, Liesenborghs L, Boudewijns R, Thibaut HJ, Dallmeier K, Velde GV, Weynand B, Beer J, Schnepf D, Ohnemus A, Remory I, Foo CS, Abdelnabi R, Maes P, Kaptein SJF, Rocha-Pereira J, Jochmans D, Delang L, Peelman F, Staeheli P, Schwemmle M, Devoogdt N, Tersago D, Germani M, Heads J, Henry A, Popplewell A, Ellis M, Brady K, Turner A, Dombrecht B, Stortelers C, Neyts J, Callewaert N, Saelens X.
Sci Transl Med. 2021 Nov 24;13(621):eabi7826
A nanobody toolbox targeting dimeric coiled-coil modules for functionalization of designed protein origami structures
Majerle A, Hadži S, Aupič J, Satler T, Lapenta F, Strmšek Ž, Lah J, Loris R, Jerala R.
Proc Natl Acad Sci U S A. 2021 Apr 27;118(17):e2021899118
A vulnerable, membrane-proximal site in human respiratory syncytial virus F revealed by a prefusion-specific single-domain antibody
Rossey I, Hsieh CL, Sedeyn K, Ballegeer M, Schepens B, Mclellan JS, Saelens X.
J Virol. 2021 Mar 10;95(11):e02279-20
Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies
Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L; VIB-CMB COVID-19 Response Team, Hoffmann M, Pöhlmann S, Graham BS, Callewaert N, Schepens B, Saelens X, McLellan JS.
Cell. 2020 May 28;181(5):1004-1015.e15
Nanobody-Mediated Neutralization Reveals an Achilles Heel for Norovirus
Koromyslova AD, Devant JM, Kilic T, Sabin CD, Malak V, Hansman GS
J Virol. 2020 Jun 16;94(13):e00660-20
Development of anti-matrix metalloproteinase-2 (MMP-2) nanobodies as potential therapeutic and diagnostic tools
Marturano A, Hendrickx MLV, Falcinelli E, Sebastiano M, Guglielmini G, Hassanzadeh-Ghassabeh G, Muyldermans S, Declerck PJ, Gresele P.
Nanomedicine. 2020 Feb;24:102103
An innovative approach in the detection of Toxocara canis excretory/secretory antigens using specific nanobodies
Morales-Yanez FJ, Sariego I, Vincke C, Hassanzadeh-Ghassabeh G, Polman K, Muyldermans S.
Int J Parasitol. 2019 Jul;49(8):635-645
Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model
Virdi V, Palaci J, Laukens B, Ryckaert S, Cox E, Vanderbeke E, Depicker A, Callewaert N.
Nat Biotechnol. 2019 May;37(5):527-530
The structure of bactofilin filaments reveals their mode of membrane binding and lack of polarity
Deng X, Gonzalez Llamazares A, Wagstaff JM, Hale VL, Cannone G, McLaughlin SH, Kureisaite-Ciziene D, Löwe J.
Nat Microbiol. 2019 Dec;4(12):2357-2368
Selective Engagement of FcγRIV by a M2e-Specific Single Domain Antibody Construct Protects Against Influenza A Virus Infection
De Vlieger D, Hoffmann K, Van Molle I, Nerinckx W, Van Hoecke L, Ballegeer M, Creytens S, Remaut H, Hengel H, Schepens B, Saelens X.
Front Immunol. 2019 Dec 12;10:2920
Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use
Lecocq Q, Zeven K, De Vlaeminck Y, Martens S, Massa S, Goyvaerts C, Raes G, Keyaerts M, Breckpot K, Devoogdt N
Biomolecules. 2019 Sep 29;9(10):548
Nanobodies and their potential applications
Hassanzadeh-Ghassabeh G, Devoogdt N, De Pauw P, Vincke C, Muyldermans S.
m
Structural Basis of Nanobodies Targeting the Prototype Norovirus
Ruoff K, Kilic T, Devant J, Koromyslova A, Ringel A, Hempelmann A, Geiss C, Graf J, Haas M, Roggenbach I, Hansman G.
J Virol. 2019 Mar 5;93(6):e02005-18
In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter
Vanmarsenille C, Elseviers J, Yvanoff C, Hassanzadeh-Ghassabeh G, Garcia Rodriguez G, Martens E, Depicker A, Martel A, Haesebrouck F, Pasmans F, Hernalsteens JP, De Greve H.
PLoS One. 2018 Sep 27;13(9):e0204222
Identification and characterization of Nanobodies targeting the EphA4 receptor
Schoonaert L, Rué L, Roucourt B, Timmers M, Little S, Chávez-Gutiérrez L, Dewilde M, Joyce P, Curnock A, Weber P, Haustraete J, Hassanzadeh-Ghassabeh G, De Strooper B, Van Den Bosch L, Van Damme P, Lemmens R, Robberecht W.
J Biol Chem. 2017 Jul 7;292(27):11452-11465
Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization
Vanmarsenille C, Díaz Del Olmo I, Elseviers J, Hassanzadeh Ghassabeh G, Moonens K, Vertommen D, Martel A, Haesebrouck F, Pasmans F, Hernalsteens JP, De Greve H.
Vet Res. 2017 Dec 8;48(1):86.
Discovery of a novel conformational equilibrium in urokinase-type plasminogen activator
Kromann-Hansen T, Louise Lange E, Peter Sørensen H, Hassanzadeh-Ghassabeh G, Huang M, Jensen JK, Muyldermans S, Declerck PJ, Komives EA, Andreasen PA.
Sci Rep. 2017 Jun 13;7(1):3385.
TNFR1 inhibition with a Nanobody protects against EAE development in mice
Steeland S, Van Ryckeghem S, Van Imschoot G, De Rycke R, Toussaint W, Vanhoutte L, Vanhove C, De Vos F, Vandenbroucke RE, Libert C.
Sci Rep. 2017 Oct 20;7(1):13646
Nanobody binding to a conserved epitope promotes norovirus particle disassembly
Koromyslova AD, Hansman GS.
J Virol. 2015 Mar;89(5):2718-30
Identification of a novel, nanobody-induced, mechanism of TAFI inactivation and its in vivo application
Hendrickx ML, Zatloukalova M, Hassanzadeh-Ghassabeh G, Muyldermans S, Gils A, Declerck PJ.
J Thromb Haemost. 2014 Feb;12(2):229-36
A nanobody modulates the p53 transcriptional program without perturbing its functional architecture
Bethuyne J, De Gieter S, Zwaenepoel O, Garcia-Pino A, Durinck K, Verhelle A, Hassanzadeh-Ghassabeh G, Speleman F, Loris R, Gettemans J.
Nucleic Acids Res. 2014 Nov 10;42(20):12928-38
A camelid antibody candidate for development of a therapeutic agent against Hemiscorpius lepturus envenomation
Yardehnavi N, Behdani M, Bagheri KP, Mahmoodzadeh A, Khanahmad H, Shahbazzadeh D, Habibi-Anbouhi M, Hassanzadeh Ghassabeh G, Muyldermans S.
FASEB J. 2014 Sep;28(9):4004-14.
Interactions between metal-binding domains modulate intracellular targeting of Cu(I)-ATPase ATP7B, as revealed by nanobody binding
Huang Y, Nokhrin S, Hassanzadeh-Ghassabeh G, Yu CH, Yang H, Barry AN, Tonelli M, Markley JL, Muyldermans S, Dmitriev OY, Lutsenko S.
J Biol Chem. 2014 Nov 21;289(47):32682-93
Stratifying fascin and cortactin function in invadopodium formation using inhibitory nanobodies and targeted subcellular delocalization
Van Audenhove I, Boucherie C, Pieters L, Zwaenepoel O, Vanloo B, Martens E, Verbrugge C, Hassanzadeh-Ghassabeh G, Vandekerckhove J, Cornelissen M, De Ganck A, Gettemans J.
FASEB J. 2014 Apr;28(4):1805-18.
In vitro and in vivo characterisation of the profibrinolytic effect of an inhibitory anti-rat TAFI nanobody
Hendrickx ML, Zatloukalova M, Hassanzadeh-Ghassabeh G, Muyldermans S, Gils A, Declerck PJ.
Thromb Haemost. 2014 May 5;111(5):824-32
Nanobody(R)-based chromatin immunoprecipitation/micro-array analysis for genome-wide identification of transcription factor DNA binding sites
Nguyen-Duc T, Peeters E, Muyldermans S, Charlier D, Hassanzadeh-Ghassabeh G.
Nucleic Acids Res. 2013 Mar 1;41(5):e59
V(H)H (nanobody) directed against human glycophorin A: a tool for autologous red cell agglutination assays
Habib I, Smolarek D, Hattab C, Grodecka M, Hassanzadeh-Ghassabeh G, Muyldermans S, Sagan S, Gutiérrez C, Laperche S, Le-Van-Kim C, Aronovicz YC, Wasniowska K, Gangnard S, Bertrand O.
Anal Biochem. 2013 Jul 1;438(1):82-9
Development of VEGFR2-specific Nanobody Pseudomonas exotoxin A conjugated to provide efficient inhibition of tumor cell growth
Behdani M, Zeinali S, Karimipour M, Khanahmad H, Schoonooghe S, Aslemarz A, Seyed N, Moazami-Godarzi R, Baniahmad F, Habibi-Anbouhi M, Hassanzadeh-Ghassabeh G, Muyldermans S.
N Biotechnol. 2013 Jan 25;30(2):205-9
TAFIa inhibiting nanobodies as profibrinolytic tools and discovery of a new TAFIa conformation
Hendrickx ML, DE Winter A, Buelens K, Compernolle G, Hassanzadeh-Ghassabeh G, Muyldermans S, Gils A, Declerck PJ.
J Thromb Haemost. 2011 Nov;9(11):2268-77
A simple quantitative affinity capturing assay of poliovirus antigens and subviral particles by single-domain antibodies using magnetic beads
Thys B, Saerens D, Schotte L, De Bleeser G, Muyldermans S, Hassanzadeh-Ghassabeh G, Rombaut B.
J Virol Methods. 2011 May;173(2):300-5
A recombinant dromedary antibody fragment (VHH or nanobody) directed against human Duffy antigen receptor for chemokines
Smolarek D, Hattab C, Hassanzadeh-Ghassabeh G, Cochet S, Gutiérrez C, de Brevern AG, Udomsangpetch R, Picot J, Grodecka M, Wasniowska K, Muyldermans S, Colin Y, Le Van Kim C, Czerwinski M, Bertrand O.
Cell Mol Life Sci. 2010 Oct;67(19):3371-87